TB-500 (Thymosin β4 fragment)
TB-500 Molecular Weight Explained
The commonly supplied TB-500 fragment has a molecular weight of approximately 4.9 kDa. Full-length Thymosin Beta-4 is approximately 4.9 kDa also (43 residues). Molecular weight is confirmed on the Certificate of Analysis by mass spectrometry as a primary identity check.
Key facts
- TB-500 MW
- ~4.9 kDa (common form)
- Verification
- Mass spectrometry on CoA
Why MW matters
Mass matching a supplier's stated sequence is the first check for identity. Deviations flag either the wrong fragment definition or impurity.
Extended research context
The TB-500 (Thymosin β4 fragment) deep dive
Deep dive: TB-500 vs full-length Thymosin Beta-4
'TB-500' is a synthetic peptide corresponding to the active 17-amino-acid actin-binding region of the endogenous 43-residue Thymosin Beta-4 protein. The two are not identical — TB-500 lacks the flanking sequence that gives full-length TB-4 additional binding partners. In the research literature, papers use 'Thymosin β4' when they mean the full protein and 'TB-500' or 'AcSDKP fragment' when they mean the shorter synthetic peptide. Reading a CoA carefully to see which molecule is in the vial matters — mass spec is the definitive check.
Actin-binding as the core mechanism
The N-terminal region of TB-4 (and TB-500 by inheritance) contains the canonical actin-binding motif. This motif sequesters G-actin monomers, modulating the G:F actin equilibrium in cell cultures. That mechanism is why almost every mechanistic paper on TB-500 traces back to cytoskeletal reorganisation, cell migration, and models of tissue repair.
Handling considerations unique to TB-500
TB-500 is a 17-residue peptide with modest amphipathicity; it reconstitutes cleanly in bacteriostatic water but is sensitive to repeated freeze/thaw. Aliquoting into single-use volumes on first reconstitution preserves potency across a batch. HPLC on the batch CoA should show a single dominant peak; a doublet suggests deamidation.
Research applications
- ▸In vitro actin-polymerisation assays (G:F actin ratio measurement)
- ▸Cell-migration and wound-scratch assays in fibroblast lines
- ▸Angiogenesis models: tube-formation and endothelial migration assays
- ▸Analytical method development for short peptides on RP-HPLC
- ▸Reference-material comparisons against endogenous Thymosin β4
Handling checklist
- ✓Store lyophilised vials at −20 °C long-term
- ✓Reconstitute with bacteriostatic water (0.9% benzyl alcohol)
- ✓Aliquot immediately to avoid freeze/thaw cycles
- ✓Refrigerate reconstituted aliquots at 2–8 °C; use within 28 days
- ✓Confirm mass (~4,963 Da for TB-500) via CoA before study use
Common research-handling mistakes
Learnt from thousands of researcher orders across our UK labs.
✗ Assuming TB-500 = full Thymosin β4
Fix: TB-500 is the 17-residue actin-binding fragment; check the CoA sequence.
✗ Repeated freeze/thaw
Fix: Aliquot at first reconstitution; each cycle degrades yield.
✗ Using tap water
Fix: Use bacteriostatic or sterile water only.
Continue researching
Peer-reviewed guides, comparators and matched reference materials.
Related questions researchers ask
- Is TB-500 the same as Thymosin Beta-4?
- How does TB-500 bind actin?
- What is the molecular weight of TB-500?
- How is TB-500 reconstituted for research?
- Is TB-500 legal to buy in the UK for research?
Frequently asked questions
- Do all TB-500 products have the same MW?
- Not necessarily — fragment definitions vary; check the CoA against the stated sequence.
Primary sources & clinical trials
Peer-reviewed research and registered trials from PubMed, ClinicalTrials.gov, PubChem, FDA and NIH. All links open in a new tab and point to the primary source, so every claim can be verified at origin.
- PubChemPubChem: Thymosin Beta-4pubchem.ncbi.nlm.nih.gov
- PubMedGoldstein et al., Thymosin β4: a multi-functional regenerative peptide — Ann NY Acad Sci 2012 (PMID 22574954)pubmed.ncbi.nlm.nih.gov
- PubMedCrockford et al., Thymosin β4 in acute myocardial infarction — Expert Opin Biol Ther (PMID 20095868)pubmed.ncbi.nlm.nih.gov
- PubMedSosne et al., Thymosin β4 corneal wound healing — Ann NY Acad Sci (PMID 20955324)pubmed.ncbi.nlm.nih.gov
- TrialClinicalTrials.gov · Thymosin β4 (RGN-259) dry eye Phase 3 (NCT03925727)clinicaltrials.gov
- PubChemPubChem · Thymosin β4 (CID 16132341)pubchem.ncbi.nlm.nih.gov
- PubMedNIH PubMed — Thymosin beta-4 tissue repairpubmed.ncbi.nlm.nih.gov
- GuidelineGoogle — Creating helpful, reliable, people-first contentdevelopers.google.com
Written and reviewed by
Jack Muncaster · Founder, UK Peptides
Jack founded UK Peptides in Manchester after repeatedly receiving research compounds with missing or recycled paperwork. He is responsible for supplier selection, batch release decisions and the content published in this research library. Every article here is sourced to primary literature and every product page to a signed third-party certificate.
More TB-500 (Thymosin β4 fragment) articles
- Thymosin Beta-4 Fragment Research ExplainedThymosin Beta-4 fragment research: why the actin-binding fragment is studied instead of the full 43-residue protein, and how findings translate.
- TB-500 Spelling and Naming VariantsTB-500 spelling variants: TB500, TB-500, Thymosin Beta-4 fragment, Tβ4, and TB4. All refer to the synthetic Thymosin Beta-4 fragment.
- What Is the Actin-Binding Domain of TB-500?The actin-binding domain of TB-500 centres on the LKKTETQ motif, a conserved sequence responsible for G-actin sequestration in the Tβ4 family.
- What Is TB-500? Research OverviewTB-500 is a synthetic peptide fragment of Thymosin Beta-4 studied for actin binding, cell migration, and angiogenesis in in vitro and animal models.
- TB-500 Peptide: Structure and OriginTB-500 peptide structure: a synthetic fragment of the 43-residue Thymosin Beta-4 protein. Sequence, origin, and relationship to native Tβ4 explained.
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