MGF

MGF in the research literature

Published work on MGF sits in three strands. The first is splicing biology. Human IGF-1 is expressed as several isoforms, and the IGF-1Ec variant (UniProt P05019-4, 158 residues) ends in the 24-residue E-domain sequence that this vial contains. Yang and Goldspink, 2002, separated that E peptide from mature IGF-1 in myoblast culture and reported the two acting on proliferation and differentiation in different ways.

The second strand is the review literature. Matheny and colleagues, 2010, kept the word putative in their title, because no dedicated receptor for the E peptide had been identified and because it was unclear whether the E domain exists as a free peptide in tissue at all. Both points remain contested.

The third strand is analytical. Cox and colleagues, 2017, characterised confiscated MGF by LC-MS, found the R23H sequence variant rather than the native peptide, and followed its breakdown in vitro. That variant is the one most research vendors still print on their sheets.

MGF vs PEG-MGF: which to order

Both vials hold the same 24-residue sequence; the difference is the polyethylene glycol chain on PEG-MGF. Plain MGF has a defined formula, C121H199N41O40, and a calculated mass of 2,868.2 Da as the free acid, so the lab report, once published on this page, will show a single mass against that figure (2,867.2 Da for the C-terminal amide). PEG is polydisperse, so a PEG-MGF report shows a broad distribution instead. Order MGF for LC-MS method work or myoblast assays, where a defined peptide matters; order PEG-MGF when the model calls for slower clearance. Check the label sequence: native MGF ends in FEERK, and FEEHK is the R23H variant. One 2mg size; packs of two, three and five take 10, 20 and 30% off. Buy MGF UK from us: same-day dispatch from Manchester, free UK delivery over £40.